Extended Data Fig. 4: Profiling myeloid cells in the aorta.

Spleen and aorta samples from (a) SNX22^gfp and (b) L-Myc^gfp reporter mice were assessed for presence of cDC1 in respective tissues, GFP (green) and Dapi (blue). In panel B aorta, sample was co-stained with CD45 (red) and MHC II (white) antibody. Aorta from XCR1-venus reporter mice were isolated and stained for MHC II (red) or CD45 (white) expression by antibody labeling, then imaged in whole-mount for presence of cDC1 in the (c) aortic arch intima, (d) adventitia, and (e) aortic valve. C57Bl/6 (WT) aorta was isolated and stained for CD103 (green), MHC II (red), and CD45 (white) by antibody labeling and imaged by confocal microscopy for cDC1 presence in the (f) intima of the aortic arch or (g) the adventitia of the aorta. Embryonic labeling was performed using CD115creER Rosa26-mTmG mice. Aorta were collected from adult animals and assessed for GFP (green) and Tomato (red) expression in mice labeled at (h) E11.5, and co-stained with CD45 (white) in mice labeled at (i) E14.5. Data are representative of (a) n = 3, (b) n = 3, (c–e) n = 6 in two experiments, (f, g) n = 6 in two experiments, (h) n = 3, and (i) n = 5 in two experiments.