Extended Data Fig. 5: Atherosclerotic plaque development in the aorta of hypercholesterolemic mice following acute depletion of MacAIR. | Nature Immunology

Extended Data Fig. 5: Atherosclerotic plaque development in the aorta of hypercholesterolemic mice following acute depletion of MacAIR.

From: Limited proliferation capacity of aortic intima resident macrophages requires monocyte recruitment for atherosclerotic plaque progression

Extended Data Fig. 5

(a) Following the schematic, CX3CR1creER CD115-stop-DTR mice were used to conditionally express DTR on MacAIR by gavage with tamoxifen, waiting three weeks to allow circulating cells to repopulation from DTR-negative progenitors. MacAIR were depleted by i.p. injection of diphtheria toxin and hypercholesterolemia induced by i.v. injection of AAV-PCSK9. Mice were started on HFD the following day. Mice were sacrificed and assessed for plaque area in the aortic arch by oil red o (ORO) staining. (b) Representative images of ORO staining and en face imaging. (c) Quantification of plaque area on the aortic arch. Data are from n = 7, Cntl and n = 4 for DTR-group and are the result of a single experiment, error bars represent SEM.

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