Fig. 1: Detection of SARS-CoV-2 structural protein antibodies by LIPS.
From: ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
a–d, Antibodies against the four SARS-CoV-2 structural proteins S (a), N (b), M (c) and E (d) were measured by LIPS from patients with COVID-19 and age-matched negative controls. e, Antibodies against the S subunits S1, S2 and S2′ by LIPS from patients with COVID-19 and negative controls. The cutoff value is shown by the dotted line and was based on the mean plus 3 × s.d. of the negative control group. Blue dots represent data points of patients with COVID-19 before day 14. f, Full S, S1, S2 and S2′ antibody LIPS responses in patients with COVID-19 with low MN titers (<160) versus high MN titers (≥160). g, Full S antibody LIPS responses in patients with COVID-19 with low ELISA S IgG responses (<1) versus high ELISA IgG responses (≥1). All data represent mean ± s.d. and individual responses (n = 15). Experiments were repeated twice. Two-sided P values were calculated using the Mann–Whitney U-test. Background values were subtracted. Asterisks indicates statistical significance between patients with COVID-19 versus negative controls or between patients grouped by MN > 160 versus MN < 160 or ELISA optical density (OD) > 1 versus OD < 1. NS, P = 0.0591, *P < 0.05, **P < 0.01.
