Extended Data Fig. 5: Immune response in the lung to HDM in mice with a Treg cell-specific deletion of ST2.
From: Interleukin-33 activates regulatory T cells to suppress innate γδ T cell responses in the lung
a–c, Il1rl1fl/flFoxp3YFPcre mice and Foxp3YFPcre littermate controls were left untreated (Naive) or were treated with HDM i.n. on days 0 and 7-11 and analyzed on day 14. a, b, Representative flow cytometry for IL-13 and IL-5 (a) and for IFN-γ and IL-17A (b) in CD4+Foxp3– (TH) cells from lung parenchyma. Data represent one experiment with n = 5 mice per group of two independent experiments in panels (a, b). c, Lung explants from Naive and HDM-treated mice were re-stimulated ex vivo with HDM and the indicated cytokines measured in culture supernatants 72 h later by ELISA. Data represent one experiment with n = 4 mice per group of two independent experiments. Unpaired two-tailed t test with Holm-Sidak correction for multiple comparisons was used for statistical analysis. Error bars denote mean ± s.d. P values are indicated in the figure.
