Extended Data Fig. 6: Innate lymphocyte response in the lung to HDM.
From: Interleukin-33 activates regulatory T cells to suppress innate γδ T cell responses in the lung
a–g, Il1rl1fl/flFoxp3YFPcre mice and Foxp3YFPcre littermate controls were left untreated (Naive) or were treated with HDM i.n. on days 0 and 7-11 and analyzed on day 14. a, Gating strategy for flow cytometric analysis of innate lymphocytes in lung parenchyma. b–d, Percentage of ILC1 (T-bet+), ILC2 (ST2+), and ILC3 (ST2–T-bet–) of CD3–Lin–CD127+ cells (b), percentage of natural killer (NK) cells of CD3–TCRαβ– cells (c), and percentage of NKT cells of CD3+TCRγδ– cells (d) in the lung parenchyma. e–g, Percentage of lung ILCs (e), NK (f), and NKT cells (g) expressing IL-13 and IL-5, IFN-γ, or IL-17A. Data represent one experiment with n = 5 mice per group of three independent experiments in panels (b–g). Unpaired two-tailed t test with Holm-Sidak correction for multiple comparisons was used for statistical analysis in panels (b–g). No statistical difference was found. Error bars denote mean ± s.d.
