Extended Data Fig. 7: Lung and airway inflammatory response to HDM and A. alternata in mice with a Treg cell-specific deletion of ST2.
From: Interleukin-33 activates regulatory T cells to suppress innate γδ T cell responses in the lung
a, b, Il1rl1fl/flFoxp3YFPcre mice and Foxp3YFPcre littermate controls were left untreated (Naive) or were treated with HDM i.n. on days 0 and 7-11 and analyzed on day 14. a, Gating strategy for flow cytometric analysis of eosinophils (CD11c–Siglec-F+), neutrophils (Siglec-F–CD11b+Ly6G+), and Ly6C+ inflammatory monocytes (Ly6C+ iMo, Siglec-F–CD11b+Ly6C+) in the lung parenchyma of HDM-treated mice. b, Quantification of cell differential counts in BAL. Mn - Mononuclear cells; Ne - Neutrophils; Eo - Eosinophils. Data represent one experiment (Foxp3YFPcre Naive n = 3; Foxp3YFPcre HDM n = 5; Il1rl1fl/flFoxp3YFPcre Naive n = 3; Il1rl1fl/flFoxp3YFPcre HDM n = 5 mice per group) of two independent experiments. Unpaired two-tailed t test with Holm-Sidak correction for multiple comparisons was used for statistical analysis. c, Il1rl1fl/flFoxp3YFPcre mice and Foxp3YFPcre littermate controls were treated with A. alternata i.n. on days 0, 1 and 17-19 and analyzed on day 20. Gating strategy for flow cytometric analysis of eosinophils and neutrophils in the lung parenchyma of A. alternata-treated mice. Error bars denote mean ± s.d. P values are indicated in the figure.
