Extended Data Fig. 8: Decreased lung CCL11 and CCL24 and allergic pulmonary inflammation in γδ T cell-deficient mice.
From: Interleukin-33 activates regulatory T cells to suppress innate γδ T cell responses in the lung
a–c, TCRγδ-deficient (Tcrd–/–) and WT mice were treated with HDM i.n. on days 0 and 7-11 and analyzed on day 14. a, Lung Ccl11 and Ccl24 mRNA levels relative to β2M determined by RT-qPCR. Data represent one experiment with n = 4 mice per group of two independent experiments. b, Number of eosinophils (CD11c–Siglec-F+) and neutrophils (Siglec-F–CD11b+Ly6G+) in lung parenchyma. c, Quantification of cell differential counts in BAL. Mn - Mononuclear cells; Ne - Neutrophils; Eo - Eosinophils. Data represent one experiment (WT n = 5; Tcrd–/– n = 6 mice per group) of two independent experiments in panels (b, c). Unpaired two-tailed t test was used for statistical analysis in panels (a, b) and unpaired two-tailed t test with Holm-Sidak correction for multiple comparisons was used for statistical analysis in panel (c). Error bars denote mean ± s.d. P values are indicated in the figure.
