Extended Data Fig. 1: Profiling ST2-expressing Treg and TH cells in the lungs of HDM-treated mice.
From: Interleukin-33 activates regulatory T cells to suppress innate γδ T cell responses in the lung
a–d, Foxp3YFPcre mice were treated with HDM i.n. on days 0 and 7-11 and analyzed on day 14. a, Schematic of experimental design. b, NanoString analysis of gene expression comparison between flow sort-purified CD4+Foxp3– T helper (TH) cells expressing ST2 (TH2) or not (ST2– TH) from lung parenchyma. Data are presented as volcano plot from three independent experiments of pooled mice (n = 7 mice per experiment). Unpaired two-tailed t test with Holm-Sidak correction for multiple comparisons was used to obtain the P values. c, Representative flow cytometry plots for IL-13 and IL-5 (top), and for IL-17A and IFN-γ (bottom) in TH2 and ST2– TH cells from lung parenchyma. d, Representative histograms showing the expression of the indicated transcription factors in ST2+ Treg cells (red) or ST2– Treg cells (blue) from lung parenchyma. Isotype control is shown in gray.
