Extended Data Fig. 2: Examination of mitochondrial phenotypes in CD8+ TILs.
From: Disturbed mitochondrial dynamics in CD8+ TILs reinforce T cell exhaustion
a, Representative electron microscope images of mitochondrial ultrastructure (arrowhead) and autophagosome-like vesicles (arrow) in CD8+ TILs. Scale bar = 500 nm. b, MDR/MGlo populations of CD8+ T cells isolated from indicated tissues of Braf/Pten mice were determined using flow cytometry (n = 12 per group). c, MDR/MGhi (red) and MDR/MGlo (blue) populations were determined using flow cytometry, followed by the quantification of the percentage of MDR/MGlo populations in activated OT-I CD8+ T cells isolated from spleens and tumors of YUMM1.7-OVA melanoma-engrafted mice (Spleen, n = 13; Tumor, n = 10). d, Mitochondrial membrane potential was measured by flow cytometry analysis on MDR intensity with or without Oligomycin A (OA) treatments on sorted MDR/MGhi and MDR/MGlo CD8+ TILs. Quantifications represent the fold changes in MDR intensity after OA treatments on indicated CD8+ TILs (n = 11 per group). All data are mean ± s.e.m. and were analyzed by two-tailed, unpaired Student’s t-test. Data are cumulative results from at least three independent experiments. Each symbol or pair represents one individual (b-d).
