Extended Data Fig. 3: Removal of α2-6 Sia linkages on B cells with Arthrobacter Uereafaciens sialidase. | Nature Immunology

Extended Data Fig. 3: Removal of α2-6 Sia linkages on B cells with Arthrobacter Uereafaciens sialidase.

From: A CD22–Shp1 phosphatase axis controls integrin β7 display and B cell function in mucosal immunity

Extended Data Fig. 3

Purified wild type B cells purified from spleen were incubated for one hour at 37 °C with Arthrobacter ureafaciens sialidase or with vehicle control (PBS, Vehicle Ctr). a,b, Flow cytometry of Arthrobacter ureafaciens-treated or vehicle control-treated WT naïve B cells (CD19+ IgD+), isolated from spleen and stained for DAPI and SNA-FITC. a, The percentage of viable cells is shown. b, The MFI of the SNA staining was expressed as a percentage of the mean MFI of the vehicle ctr-treated WT B cell group. Representative histogram overlay gated in live naïve B cells is shown.

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