Extended Data Fig. 4: CD22-deficient T cells display wild-type levels of tyrosine phosphorylation in cell surface β7.
From: A CD22–Shp1 phosphatase axis controls integrin β7 display and B cell function in mucosal immunity
Left panel: detection of β7 and phosphotyrosine (pTyr) levels in the cell surface and intracellular β7 fractions of wild-type (WT) and CD22-deficient (Cd22–/–) T cells after the double IP as shown in Fig. 5a. Right panel: quantification of pTyr levels normalized to β7 levels (pTyr/β7 ratio). Within each experiment, the pTyr/β7 of the cell surface β7 of the WT group was set to 100, and data expressed as a percentage of this total. Each dot represents one independent experiment with n = 4 animals pooled for WT and Cd22–/– (that is n = 8 animals total for the two experimental replicates).
