Extended Data Fig. 10: Infected macrophages trigger MAIT cells activation in vitro.

PBMCs of uninfected patients were challenged with infected macrophages by the SARS-CoV-2 virus. a, Graphical flowchart of the in-vitro co-culture experiments. b, Representative dot plot and frequency of CD69⁺ MAIT cells in healthy donors (n = 4) co-cultured with different SARS-CoV-2 Multiplicity of Infection (MOI) with mock, 0.3, and 3, with replicates. Each symbol represents the mean (± SEM) value of replicates. c, Viral quantification of SARS-CoV-2 in co-culture from four donors at 96 h, by RT-qPCR, with replicates (donor 1 and 2, n = 2, donor 3 and 4, n = 3). d,e, Frequency of CD69⁺ cells among MAIT cells, conventional CD8⁺ and CD4⁺ T cells, γδT, NK, and ILC3 cells in donor n°4 (d) and all donors (e) co-culture at 96 h, with replicates (n = 2-4). Barplots represent mean (± SD) values. f, Co-culture of infected macrophages (MOI 3) with PBMC (donor 4) or purified T cells (donor 5) with or without anti-IL-12, anti-IL-18, or recombinant soluble IFN receptor protein (B18R), with replicates (n = 2-4). Each symbol represents a single co-culture well. Illustration with images from Servier Medical Art, licensed under the Creative Commons Attribution 3.0 Unported License.