Extended Data Fig. 1: Models of myeloid cell differentiation in WT and Irf8−/− mice.
From: A RUNX–CBFβ-driven enhancer directs the Irf8 dose-dependent lineage choice between DCs and monocytes
Irf8 starts to be expressed at the MPP stage and its expression sharply increases in MDPs. The expression of Irf8 further increases as cells differentiate into the DC lineage, while remaining relatively low or downregulated in the monocytic lineage. Neutrophils do not express Irf8. Irf8–/– mice lack Ly6C+ monocytes, CDPs, pDCs, and cDC1s. Irf8–/– mononuclear phagocyte progenitors accumulate and aberrantly give rise to neutrophils. MPPs include both MPP3s and MPP4s/LMPPs that express low amounts of Irf8. The dashed lines denote disputed pathways. Cell populations affected by the enhancer or gene deletion are highlighted in green.
