Extended Data Fig. 3: NLRX1 is upregulated in HIV-1 infected CD4 T cells.
From: Multi-omics analyses reveal that HIV-1 alters CD4+ T cell immunometabolism to fuel virus replication
a, Jurkat cells were infected with VSV-G-pseudotyped HIV NL4-3-EGFP virus (MOI = 0.5). After 48 hours post-infection (hpi), cells were stained with an anti-NLRX1 antibody and subjected to ImageStreamX MkII imaging flow cytometry. GFP positive cells indicate HIV-1 infection. b, The mean fluorescence intensity (MFI) of NLRX1 staining in GFP negative (n = 532 cells) and positive cells (n = 222 cells). P value was calculated by two-tailed unpaired Student’s t-test. c, The linear relationship between the expression of NLRX1 and GFP. R, Pearson’s correlation coefficient; Correlation is significant at P < 0.0001. d, Jurkat cells were infected by VSV-G-NL4-3-EGFP pseudovirus (MOI = 1). At 48 hpi, NLRX1 transcripts were assessed by qPCR. n = 3 cell cultures per experiment. P value was calculated by two-tailed unpaired Student’s t-test. e, NLRX1 transcripts were assessed in human primary CD4 T cells infected by three different HIV-1 clinical isolates at 48 hpi by qPCR. n = 3 cell cultures per experiment. Significance was tested by one-way ANOVA followed by Dunnett’s multiple comparisons test. All data are representative of three independent experiments. Error bar is s.e.m.
