Extended Data Fig. 2: Metabolic inhibitors suppress HIV-1 replication in CD4 T cells and NRG-hu CD4 mice.
From: Multi-omics analyses reveal that HIV-1 alters CD4+ T cell immunometabolism to fuel virus replication
a, The effect of OXPHOS inhibitors on virus replication in VSV-G-NL4-3-Luc-infected Jurkat cells. Rotenone (0.2 µM or 1 µM), metformin (1 mM or 5 mM, mitochondrial complex I inhibitor) and antimycin A (0.2 µM or 1 µM, mitochondrial complex III inhibitor). Ethanol is the vehicle control for rotenone and antimycin A, and sterile water is the vehicle control for metformin. n = 3 cell cultures per experiment. b, The oxygen consumption rate (OCR) was measured in Jurkat cells infected with VSV-G-NL4-3-Luc or left uninfected (mock) in the presence of metformin or the vehicle control (H2O). n = 4 cell cultures per experiment. c, The basal and maximal OCR and reserved respiratory capacity of Jurkat cells infected with VSV-G-NL4-3-Luc or left uninfected (mock) in the presence of metformin or vehicle control (H2O). n = 12 cell cultures per experiment. d, The effect of 2-DG (5 mM or 10 mM), a glycolysis inhibitor, on virus replication in VSV-G-NL4-3-Luc infected Jurkat cells. H2O was used as vehicle control. n = 3 cell cultures per experiment. e, Representative FACS plots of human CD4 T cells from peripheral blood stained with the indicated markers. Cells were obtained from mock or HIV-1 R3A infected NRG-hu CD4 mice with or without metformin treatment. Data are representative of three independent experiments shown as the mean ± s.e.m. Statistical significance was tested by one-way ANOVA (a,d) or two-way ANOVA followed by Tukey’s multiple comparisons test (c).
