Extended Data Fig. 2: Gating strategy for Intracellular cytokine staining. | Nature Immunology

Extended Data Fig. 2: Gating strategy for Intracellular cytokine staining.

From: Robust SARS-CoV-2-specific T cell immunity is maintained at 6 months following primary infection

Extended Data Fig. 2

First, lymphocytes were gated according to FS-A and SS-A before removing doublets according to FS-A and FS-H. Live T cells were then gated using an amine reactive red fixable viability dye and anti-CD3. Finally, CD4 + and CD8 + T cells were gated according to positive staining with anti-CD4 and anti-CD8, respectively.

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