Extended Data Fig. 3: Impaired TAP function blocks classic MHC-I presentation but not cross-presentation.
From: TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming
a, Classic MHC-I presentation by WT DCs of SIINFEKL derived from influenza PR/8-OTI virus to OVA-specific CD8+ T cells. DCs were infected with influenza PR/8-OT-I virus for 5h and incubated in the presence of active or inactive US6. b, c, WT and Tap−⁄− DC cross-presentation of SIINFEKL from E. coli-OVA at several time points (b), or at 4.5h post-phagocytosis of either E. coli-OVA or 5h post-infection with influenza PR/8-OTI virus (c). Data represent at least three independent experiments except for (b) depicting two independent experiments. The mean ± SEM are presented and each symbol represents biological replicate. **P < 0.01; N.S.= non statistically significant (P>0.05) using an unpaired two-tailed t-test.
