Extended Data Fig. 4: Classic MHC-I presentation of viral antigen by Wt DC is not affected by Sec22b or Rab11a inhibition.
From: TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming
a, Immunoblots for the expression of β-actin, Sec22b and Rab11a on whole cell extracts prepared from DCs seven days post-differentiation of bone marrow progenitors that had been transduced with lentiviruses encoding for control shRNA or shRNA targeting either Rab11a or Sec22b. Immunoblots were cropped to show indicated proteins. b, WT DC progenitors were transduced with recombinant lentiviruses expressing scrambled, Sec22b or Rab11a specific shRNA. Classic MHC-I presentation of SIINFEKL at 5h after infection with recombinant SIINFEKL-expressing Influenza-OTI (PR/8-OTI) (left panels, no secondary treatment) or cross-presentation of SIINFEKL from heat-inactivated Influenza-OTI given to DCs at 3h following infection by Influenza-OTI virus. Cross-presentation was assessed 2h later. Data represent at least three independent experiments. The mean ± SEM are presented and each symbol represents biological replicate. N.S.= non statistically significant (P>0.05) using an unpaired two-tailed t-test.
