Extended Data Fig. 7: Interactions between Grem1+ FRCs and DCs.
From: Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues
a, Left: Uniform Manifold Approximation and Projection (UMAP) plot visualization of 12,620 murine dendritic cell sorts (dots) colored by cluster identity. Right: UMAP as on the left, here colored by expression of indicated marker genes. b, Average relative expression of the 10 most strongly upregulated genes (by LogFC, rows) across clusters from (a). Two representative genes per cluster are highlighted. c, Fold change in expression of Flt3l mRNA in indicated cell types from skin draining lymph nodes of Grem1-Cre-ERT2 Rosa26 EYFP/iDTR mice with respect to Grem1-Cre-ERT2 Rosa26 EYFP mice upon DTxn mediated ablation, results are normalized to those of the gene encoding Rpl19 (ribosomal protein L19). d, Quantification of FLT3L by ELISA of total lymph node protein lysates from skin draining lymph nodes of Grem1-Cre-ERT2 Rosa26 EYFP and Rosa26 EYFP/iDTR mice upon DTxn mediated ablation. e, Left: UMAP plot visualizing 16,226 human CD11c + cells form lymph nodes of three patients colored by cluster identity. Middle: UMAP as on the left, here colored by expression of marker genes. Right: Average relative expression of the 10 most strongly upregulated genes (by LogFC, rows) in clusters from the left. f, Percentage of cells from each patient in each of the clusters from Fig. 7f. g, Enrichment analysis of ligands expressed by GREM1+ FRCs predicted to be involved in paracrine signaling events with DC1. (c) data are pooled from three independent experiments; (d) data are pooled from three independent experiments (YFP n = 14; YFP/iDTR n = 13). **P < 0.01 (two-tailed unpaired Student’s t-test). Exact P-values in extended source data. Data are shown as mean ± SEM.
