Extended Data Fig. 7: Gating strategy for the assessment of HO-1 in peripheral blood monocyte subsets.
From: Heme catabolism by tumor-associated macrophages controls metastasis formation
a, Monocytes from peripheral blood samples were identified as CD45+ CD3− CD19− CD56− cells after doublet exclusion, live cell selection and FS-A vs. SS-A gating (upper set of dot plots). Intracellular HO-1 expression (geometric mean fluorescence intensity, gMFI) detected in permeabilized cells was then recorded in 15 monocyte subsets (R1 to R3 and R1R5 to R3R8) defined by differential expression of CD14, CD16, CD88 and Tie-2. b, Median HO-1 gMFI values for each of the 15 monocyte subsets observed in peripheral blood samples from the discovery, confirmation and combined sets (discovery + confirmation). c, Expression of HO-1 (gMFI) in main monocyte subsets in peripheral blood samples from the discovery (n = 47) and confirmation (n = 45) sets of AJCC stage IIIC and IV melanoma patients. Statistical analysis of the combined set (discovery+confirmation cohorts) gMFI HO-1 values by Kruskal Wallis test followed by Dunn’s multiple comparisons test. ns= not significant; *: P < 0.05; **: P < 0.01; ****: P < 0.0001.
