Extended Data Fig. 9: Prognostic significance of myeloid-specific HO-1.
From: Heme catabolism by tumor-associated macrophages controls metastasis formation
a, expression of CD163 and HO-1 in metastatic lesions of AJCC Stage III melanoma patients. Consecutive sections from lesions (LN=lymph node; s.c.: subcutaneous) of 10 patients were stained for CD163 and HO-1. Wide field images and higher magnification insets are shown centered on the same area of the tumor tissue for both markers. Lesions from patients #1 to #8 (short survival) show expression of both CD163 and HO-1 in the same areas of the tumor tissue. Lesions from patients #11 to #15 (long survival) show lack of both CD163 and HO-1 expression. For each patient, survival was computed from the date of surgery for removal of metastatic lesions. Status: DOD: dead of disease, NED: no evidence of disease. b, results of multivariable Cox proportional hazard model on TCGA skin cutaneous melanoma dataset (n = 471) were obtained through the outcome module of TIMER2.0 web server, by testing the relevance of tumor infiltrating macrophages, as identified by the M2_TIDE macrophage signature52, and of the expression of macrophage-related genes CD163, HO-1 and CD68. Tumor stage was included as clinical covariate.
