Extended Data Fig. 2: Impact of F4/80hi TAMs accumulation on metastasis formation and tumor angiogenesis.
From: Heme catabolism by tumor-associated macrophages controls metastasis formation
a, area of metastatic burden quantified from H&E stained lung sections from WT MN-MCA1-bearing mice treated with vehicle or anti-CSF1R is expressed as a percentage of total lung surface area. Representative images are shown. Scale bars, 1 mm. n = 4 (Vehicle) and n = 5 (anti-CSF1R), one experiment was performed. b, FACS quantification of CD31+ cells in lung metastasis of WT MN-MCA1-bearing mice treated with vehicle or anti-CSF1R; n = 5 (Vehicle) and n = 4 (anti-CSF1R), shown are data representative of two independent experiments. c, Representative H&E stained lung sections from WT and C3–/– MN-MCA1-bearing mice and quantification of area of metastatic burden expressed as a percentage of total lung surface area. Scale bars, 1 mm. n = 3 (Vehicle) and n = 5 (C3–/–), one experiment was performed. d, FACS quantification of CD31+ cells in lung metastasis of WT and C3–/– MN-MCA1-bearing mice; n = 5 (Vehicle) and n = 4 (C3–/–), shown are data representative of two independent experiments. e, Representative images of MN/MCA1 tumor sections from WT, C3–/– and anti-CSF1R treated mice injected with FITC-Dextran. Sections were co-stained for FITC-Dextran (green) and CD31 (red) (top left); quantification of vascular permeability expressed as percentage of colocalization rate between extracted signal of CD31+ and FITC+ pixels (bottom left). Representative images of MN/MCA1 tumor sections from WT, C3–/– and anti-CSF1R treated mice costained for collagen IV (green), CD31 (red) and α-SMA (white) (top right); quantification of vessel stability was assessed as colocalization rate of Collagen IV+ or α-SMA+ with CD31+ vessels (bottom right); scale bars, 10 μm; n = 3 mice per genotype, one experiment was performed. Data are mean ± SEM. *P < 0.05 **P < 0.01 ***P < 0.001, ****P < 0.0001 between selected relevant comparisons. In a-d, two-tailed unpaired Student’s t-test. In e, one-way ANOVA and Kruskal-Wallis test with Dunn’s multiple comparisons test.
