Extended Data Fig. 1: Lineage-associated gene signatures of CD45+ and CD45– SVF cells from healthy human WAT. | Nature Immunology

Extended Data Fig. 1: Lineage-associated gene signatures of CD45+ and CD45– SVF cells from healthy human WAT.

From: Single-cell sequencing of human white adipose tissue identifies new cell states in health and obesity

Extended Data Fig. 1

a, Dot plot showing selected top differentially expressed marker genes for each cluster, supporting assignment of clusters to compartments shown in Fig. 1b. Color saturation indicates the strength of expression in positive cells, while dot size reflects the percentage of each cell cluster expressing the gene. b, Violin plots showing expression levels of additional cluster markers for the indicated structural cell populations. c, Representative gating strategy for scRNAseq-defined human WAT non-immune cell populations (CD45–): Endothelial cell: CD31+, Smooth muscle cell (SMC): CD31–CD34–CD29–, Adipocyte precursor cell (APC): CD31–CD34–CD29+, Preadipocyte: CD31–CD34+CD29intICAM-1+CD26–, Interstitial progenitor cell: CD31–CD34+CD29intICAM-1+CD26+. d, Density correlation analysis of the indicated non-immune subsets with patient BMI. Line of best fit and 95% confidence intervals are shown for each plot. Each point represents an individual patient. Linear regression and two-tailed Pearson Correlation analysis with 95% confidence intervals were conducted. p < 0.05 was considered significant.

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