Extended Data Fig. 4
Diphtheria toxin (DT) administered from day 8 to 10 (DT8-10) after birth followed by quantitative analyses on day 11 (H11) of the absolute count of macrophages (CD45+ Lin− F4/80+ CD64+) in the colon (a) or spleen (b) and the absolute count of iNKT (CD45+ CD3ε+ TCRβ+ CD1d Tetramer+) and TCR-αβ+ T (CD45+ CD3ε+ TCRβ+) cells in the colon (c) and spleen (d) of control littermates Cx3cr1+/- (n = 4) or Cx3cr1DTR (n = 7) mice. e) Schematic of macrophage depletion model with AFS98 antibody. AFS98 or Isotype control antibody administered from day 4 to 10 (AFS4-10) after birth followed by quantitative analyses on day 11 (H11) of the absolute count of macrophages (n = 6 per group) (f), and the absolute count of iNKT and TCR-αβ+ T cells (n = 12 per group) (g) in the colon of injected animals. Absolute counts were determined by flow cytometry. Error bars indicate standard error of mean. Each dot is representative of an individual mouse. P values were calculated by unpaired two-sided Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: not-significant.
