Extended Data Fig. 8

a, Schematic of macrophage depletion model ex vivo. Adult spleen from control littermates LysCre^+/- or MM^DTR animals, digested and cultured for 48 hours with Diphtheria toxin (DT) followed by quantitative analyses of the absolute count of macrophages (CD45⁺ Lin⁻ F4/80⁺ CD64⁺, LysCre^+/-: n = 4, MM^DTR n = 5) (b), and the absolute count of iNKT (CD45⁺ CD3ε⁺ TCRβ⁺ CD1d Tetramer⁺) and TCR-αβ⁺ T (CD45⁺ CD3ε⁺ TCRβ⁺) (LysCre^+/-: n = 5, MM^DTR n = 5) cells (c). d, Representative plot of macrophages on day 49 in the colon of control littermates LysCre^+/- (n = 4) or MM^DTR (n = 4) animals treated with DT from day 8 to 14 after birth. DT administered from day 8 to 14 (DT8-14) after birth followed by quantitative analyses on day 49 (H49) of the absolute count of macrophages (e), and the absolute count of iNKT and TCR-αβ⁺ T cells in the skin (F) of control littermates LysCre^+/- or MM^DTR animals. Absolute counts were determined by flow cytometry. Error bars indicate standard error of mean. Each dot is representative of an individual mouse. P values were calculated by unpaired two-sided Student’s t-test. *P < 0.05, ***P < 0.001, ns: not-significant. Representative plot of TCR-αβ⁺ T and iNKT cells on day 49 in the colon of control littermates LysCre^+/- or MM^DTR animals treated with DT from day 8 to 14 after birth in the colon (g) and spleen (h). Unl, Unloaded. Tet, Tetramer. Numbers in the representative plots indicate cell frequency.