Extended Data Fig. 9

Representative plot (left) and cell percentage (right) of iNKT cell subsets (NKT1, NKT2, NKT 17) from 42 day old control littermates LysCre^+/- (n = 6) or MM^DTR (n = 4) animals treated with DT from day 8 to 14 (DT8-14) after birth in the colon (a) or spleen (b). Mean fluorescent intensity (MFI) of Ki67, percentage of Ki67⁺ and CD69⁺ TCR-αβ⁺ T (CD45⁺ CD3ε⁺ TCRβ⁺) and iNKT (CD45⁺ CD3ε⁺ TCRβ⁺ CD1d Tetramer⁺) cells on day 42 (H42) in the colon (c,d,g) or spleen (E,F,H) of control littermates LysCre^+/- (n = 6) or MM^DTR (n = 4) animals treated with diphtheria toxin (DT) from day 8 to 14 after birth. I) Schematic of macrophage depletion model and αGalactosylceramide (αGal) treatment. DT administered from day 8 to 14 after birth followed by αGal regimen on day 49 and quantitative analyses 16 hours after, of the IFNγ protein level in the colon of control littermates LysCre^+/- (n = 10) or MM^DTR (n = 8) animals by enzyme-linked immunosorbent assay (ELISA) (J). DT administered from day 8 to 14 after birth followed by Listeria monocytogenes administration by oral gavage on day 49 and analyses of Ifnγ (K) or Il12p40 (L) mRNA expression in the colon of control littermates LysCre^+/- (n = 6) or MM^DTR (n = 6) animals 3 days after infection by quantitative polymerase chain reaction analysis. Numbers in the representative plots indicate cell frequency and were determined by flow cytometry. Error bars indicate standard error of mean. Each dot is representative of an individual mouse. P values were calculated by unpaired two-sided Student’s t-test. *P < 0.05, **P < 0.01, ns: not-significant. Numbers in the representative plots indicate cell frequency.