Extended Data Fig. 2

Diphtheria toxin (DT) administered every two days from day 8 to 14 (DT8-14) after birth followed by quantitative analyses on day 15 (H15) of the absolute count of macrophages (CD45⁺ Lin⁻F4/80⁺ CD64⁺) in the skin (LysCre^+/-: n = 3, MM^DTR n = 4) (a) or spleen (LysCre^+/-: n = 5, MM^DTR n = 5) (b) and the absolute count of iNKT (CD45⁺ CD3ε⁺ TCRβ⁺ CD1d Tetramer⁺) and TCR-αβ⁺ T (CD45⁺ CD3ε⁺ TCRβ⁺) cells in the skin (LysCre^+/-: n = 8, MM^DTR n = 7) (c) of control littermates LysCre^+/- or MM^DTR animals. DT administered from day 8 to 10 (DT8-10) after birth followed by quantitative analyses on day 11 (H11) of the absolute count of iNKT and TCR-αβ⁺ T cells in the small intestine (d) and lung (e) of control littermates LysCre^+/- (n = 3) or MM^DTR (n = 12) animals. DT administered from day 8 to 10 (DT8-10) after birth followed by quantitative analyses on day 11 (H11) of the absolute count of splenic macrophages (f) of control littermates LysCre^+/- (n = 5) or MM^DTR (n = 3) animals. DT administered from day 12 to 14 (DT12-14) after birth followed by quantitative analyses on day 15 (H15) of the absolute count of splenic macrophages (g) of control littermates LysCre^+/- (n = 9) or MM^DTR (n = 10) animals. Absolute counts were determined by flow cytometry. Error bars indicate standard error of mean. Each dot is representative of an individual mouse. P values were calculated by unpaired two-sided Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.