Extended Data Fig. 3: RGS1 upregulation in CTLs and TH1 cells correlates with the weak recruitment ability.
a, Representative images and the quantification of RGS1, RGS2 and RGS16 in naïve and effector T cells (mean±s.e.m., n = 6). Scale bar, 5 μm. b, Expression of RGS1, RGS2 and RGS16 was performed by RT-qPCR and normalized to GAPDH in TH1, TH2 and effector CD8+ T cells (CTLs) isolated from peripheral blood of healthy donors (mean±s.e.m., n = 5). c, Representative histograms of RGS1 expression in TH1, TH2 and effector CD8+ T cells isolated from peripheral blood of healthy donors, determined by flow cytometry. The bars correspond to the mean fluorescence intensity (MFI) of RGS1 (mean±s.e.m. × 104, n = 5). d, Expression of RGS1, RGS2 and RGS16 was performed by RT-qPCR and normalized to GAPDH in TH1 and CTLs isolated from breast cancer with low or high TI-TH1/CTLs (mean±s.e.m., n = 4). e, First row: automated identification of the cells co-stained with CD8 and CD45RO by Imaris software to built CD8+CD45RO+ cells (yellow). Second row: automated identification of the cells co-stained with CD4 and T-bet by Imaris software to built CD4+T-bet+ cells (yellow). The mean intensity of RGS1 in these cells were analyzed by ImageJ. Scale bar, 50 µm. f, Representative histograms of RGS1 expression in TH1, TH2 and CTLs isolated from peripheral blood of breast cancer patients, determined by flow cytometry. The bars correspond to MFI of RGS1 (mean±s.e.m. × 104, n = 5). g, CTL/TH1 infiltration is positively associated with the apoptosis of breast cancer cells (TUNEL+ %) (n = 46, Pearson’s correlation coefficient R and two-tailed P value). h, CTL infiltration, but not TH1, is negatively correlated to the proliferation of tumor cells (Ki-67+ %) (n = 46, Pearson’s correlation coefficient R and two-tailed P value). The exact P values are listed in the graph (a, c, d, f, g, h). ****P < 0.0001 (b, f). P values were determined by two-tailed Student’s t-test (a, d) and two-tailed one-way ANOVA with Tukey’s multiple comparison test (b, c, f).
