Extended Data Fig. 4: RGS1 impairs CTL and TH1 recruitment in NSCLC patients.
a,b, Representative immunofluorescent staining for RGS1 expression of CTLs (a) and TH1 cells (b) infiltrated in tumors and lymph nodes (LN) indicated by arrows in human non-small cell lung cancer (NSCLC) with low or high TI-CTL/TH1 (CD4/CD8-white, T-bet/CD45RO-red, RGS1-green) (n = 74). Asterisk indicates the location of higher magnification images at right. Scale bar, 50 μm. The mean fluorescent intensity of RGS1 in CTLs (a) and TH1 cells (b) is shown on the right, analyzed by Image J software (mean±s.e.m., low TI-TH1, n = 37; high TI-TH1, n = 37; low TI-CTL, n = 37; high TI-CTL, n = 37). The exact P values are listed in the graph. c,d, Representative histograms of RGS1 expression in CTLs (c) and TH1 (d) isolated from LN, peripheral blood (PB), and tumors (T) of patients with low or high TI-CTL/TH1, determined by flow cytometry. The bars correspond to the mean fluorescent intensity (MFI) of RGS1 (mean±s.e.m. × 104, n = 5). *P < 0.05, **P < 0.01. The exact P values are as follow: P = 0.0070 (CTL in LN), 0.0133 (CTL in PB), 0.0062 (CTL in T), 0.0106 (TH1 in LN), 0.0055 (TH1 in PB), 0.0294 (TH1 in T). e, High RGS1 expression in CTLs or TH1 cells in LN of NSCLC patients, determined by mean fluorescent intensity, is associated with less tumor-infiltrating CTLs or TH1 cells (n = 74) (Pearson’s correlation coefficient R and two-tailed P values). f, Kaplan-Meier survival curves for OS in NSCLC patients with high (n = 37) and low (n = 37) RGS1 expression in CTLs or TH1 cells in LN, determined by the median value of MFI evaluated by immunofluorescent staining. The exact P values are listed in the graph (a-b, c, d, f, g, h). ****P < 0.0001 (b, f). P values were determined by two-tailed Students’ t-test (a-d).
