Extended Data Fig. 1: IL-10–Fc promotes tumor infiltration of T cells but shows less effects on other immune cells.
From: Metabolic reprogramming of terminally exhausted CD8+ T cells by IL-10 enhances anti-tumor immunity
a, Schematic diagram of the production and purification of IL-10–Fc. b, Representative size-exclusion chromatographic traces. Peak 3 (P3) was collected and analyzed. c, Representative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of purified IL-10–Fc. DTT, dithiothreitol. Results are one representative of three independent experiments. d, Splenocytes from PMEL mice were cultured with human gp10025–33 (hgp100) peptide (1 μM) and IL-2 (10 ng ml−1) for 3 d in the presence of IL-10–Fc or recombinant IL-10 at a series of concentrations. Shown are fold changes of CD8+ T cell counts (normalized by that in PBS control) at each concentration of cytokine. e-i, The experimental setting was the same as described in Fig. 1. Data are one representative of three or four independent experiments. e, Counts of CD45.2+ tumor infiltrating lymphocytes (TILs) and CD3+ TILs in B16F10 tumors. Shown are pooled data of two independent experiments (n = 10 independent animals). f, Representative immunofluorescence images of samples from each group. Green, CD3; blue, DAPI. Scale bar: 100 μm. Results are one representative of two independent experiments. g, Counts of various immune cell subsets in B16F10 tumors. For endogenous CD8+ T cells, PMEL CD8+ T cells, and total CD4+ T cells, shown are pooled data of two independent experiments (n = 10 independent animals). For other immune cells, data are representative of three independent experiments (n = 5 independent animals). NK: natural killer; DCs: dendritic cells; TAMs: tumor associated macrophages; MDSCs: myeloid-derived suppressive cells. h, Mean fluorescence intensity (MFI) of maturation markers on CD11b+CD11c+ tumor infiltrating DCs (n = 5 independent animals). i, Frequencies of M1 phenotype (CD206–MHC-II+) among CD11b+F4/80+ TAMs (n = 5 independent animals). All data represent the mean ± s.e.m. and are analyzed by two-sided Student’s t-test or one-way ANOVA and Tukey’s test; NS, not significant (P > 0.05).
