Extended Data Fig. 2: Transcriptome analysis of murine TFR cells and characterization of TFR cells in murine tumors.
From: Intratumoral follicular regulatory T cells curtail anti-PD-1 treatment efficacy
a, Schematic of immunization model in which mice were immunized intraperitoneally (i.p.) with Ovalbumin in complete Freund’s adjuvant, Ovalbumin in Monophosphoryl Lipid A or mock PBS. b, tSNE plot of TEFF (CD19–CD45+CD3+CD4+CXCR5−GITR–CD25–CD62L–CD44+), TREG (CD19–CD45+CD3+CD4+CXCR5–GITR+CD25+), TFH (CD19–CD45+CD3+CD4+CXCR5+GITR–) and TFR (CD19–CD45+CD3+CD4+CXCR5+GITR+). Each symbol represents data from an individual mouse sample (n = 9 for TEFF, n = 11 for TREG, n = 11 for TFH, n = 11 for TFR) that passed quality controls. c, Euler diagrams show the overlap of differentially expressed genes (left, upregulated in TFR, right, downregulated in TFR) in TFR cells compared to the indicated cell types. d, Heatmap comparing gene signatures of TEFF, TREG, TFH and TFR cells. Depicted are transcripts that change in expression more than 2-fold with a DEseq2 adjusted-P value of ≤ 0.05. e, Log-transformed RNA-seq expression values for each of the indicated differentially expressed genes. Each symbol represents an individual sample, data are mean + /− s.e.m. f, Representative histogram plot showing MFI of the surface expression of indicated markers in human tumor-infiltrating TFR cells (n = 4).
