Extended Data Fig. 6: RUNX1 deficiency impairs neutrophil maturation.
From: Distinct transcription factor networks control neutrophil-driven inflammation
a, Irf5 deficiency does not affect neutrophil maturation. Left: representative flow cytometry of neutrophil subsets (pre-neutrophils, immature and mature neutrophils) from WT and Irf5−/− mice. b, JunB deficiency does not affect neutrophil maturation. Left: representative flow cytometry of neutrophil subsets (pre-neutrophils, immature and mature neutrophils) from JunBfl/fl and S100a8cre/crexJunBfl/fl mice. Right: Statistical analysis of percentages of indicated neutrophil subsets. Data are shown as means and SD derived from three mice from each group. Statistical comparison was made by ordinary one-way ANOVA with Dunnett’s multiple comparisons test: ns, no significant difference. c, Absolute quantification of neutrophil subsets (pre-neutrophils, immature and mature neutrophils) in the bone marrow (left) and the blood (right) from Runx1fl/fl and Lyz2Cre/cre xRunx1fl/fl mice. d, Morphology assessment of CD11b+Ly6G+ neutrophils sorted from Runx1fl/fl and Lyz2Cre/crexRunx1fl/fl mice. e, Morphology assessment of CD11b + Ly6G+ neutrophils sorted from JunBfl/fl and S100a8cre/crexJunBfl/fl mice. (d, e) Results are expressed as percentages of segmented, banded neutrophils and metamyelocyte out of at least 200 cells counted from different fields and independent replicates. Scale bars represent 10 µm. a, c, d, e) Data are shown as means and SD from three mice from each group within one experiment. Significant differences compared between two individual groups are denoted as: *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (RM two-way ANOVA with Šidák’s multiple comparisons test). f, Differential expression of Cebpe in CD11b+Ly6G+ neutrophils sorted from Runx1fl/fl and Lyz2Cre/crexRunx1fl/fl mice, measured by flow cytometry. Gating strategy (top) of neutrophils for identifying CD11b+Ly6G+ neutrophils. Fluorescence histogram (middle) and geometric mean (bottom) of CEBPE expression in CD11b+Ly6G+ neutrophils. Data are shown as means and SD derived from three mice from each group. Significant differences compared with Runx1fl/fl and Lyz2Cer/crexRunx1fl/fl mice are denoted as: **P < 0.01 (unpaired student t test).
