Extended Data Fig. 1: Ablation of anti-silencer does not substantially disrupt development of class II-restricted thymocytes. | Nature Immunology

Extended Data Fig. 1: Ablation of anti-silencer does not substantially disrupt development of class II-restricted thymocytes.

From: Essential role of a ThPOK autoregulatory loop in the maintenance of mature CD4+ T cell identity and function

Extended Data Fig. 1

a, Flow cytometric analysis of CD4, and CD8a expression in total thymocytes (top row), or gated mature (TCRβ + CD69- CD24- CD62L + ) thymocytes of WT mice and homozygous ThPOKΔOB11/ΔOB11 mice, as indicated. A total of 4 animals of each genotype were analyzed over 2 independent experiments. Plots at right indicate % of SP CD4, CD8, and CD4 + 8 + (DP) thymocytes among total thymocytes (top panel), or gated mature thymocytes (as defined in panel a) (n = 4, for each strain). b, RNA was collected from freshly sorted thymocyte subsets, as indicated, before probing for ThPOK expression by qPCR. Data represent 2 technical replicates, each derived from pooled RNA of 3 animals. Data are presented as mean values + /- SEM. Experiment was repeated 3 times. c) Flow cytometric analysis of TCRβ and CD69 expression in total thymocytes (top row), or gated mature thymocytes of β2m-/- ThPOK + /+ and β2m-/- ThPOKΔOB11/ΔOB11 mice, as indicated. Data are representative of 6 animals of each genotype that were analyzed in 3 separate experiments.

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