Extended Data Fig. 2: Efficient inducible deletion of targeted transcription factors in Id2creERT2, Id2iΔRORγt, Id2iΔRORγt/RORα and Id2iΔRORγt/Tbet mice.
From: Reciprocal transcription factor networks govern tissue-resident ILC3 subset function and identity
a Representative histograms showing expression of RORγt (upper row) and T-bet (lower row) by tdRFP+KLRG1- ILCs from SILP of Id2creERT2, Id2iΔRORγt, Id2iΔRORγt/RORα and Id2iΔRORγt/Tbet mice, dotted line denotes positive/negative staining. b Proportion of tdRFP+KLRG1- ILCs expressing RORγt and T-bet in Id2creERT2 (n=20), Id2iΔRORγt (n=13), Id2iΔRORγt/RORα (n=8) and Id2iΔRORγt/Tbet (n=17) mice (Data are representative of 7 independent experiments). c Schematics of Rorc and Rora cDNA showing exons, the location of LoxP sites and the qPCR primer annealing sites, floxed exons removed by cre-mediated deletion are in light grey. Relative expression of transcripts for the exon3-exon4, exon7-exon8 and exon8-exon9 junctions of the Rorc locus (d) and for the exon3-exon4, exon7-exon8 and exon9-exon10 junctions of the Rora locus (e) as depicted in ‘c’, determined by qPCR analysis of cDNA prepared from tdRFP+ ILCs sorted from SILP of Id2creERT2 (n=5), Id2iΔRORγt (n=7), Id2iΔRORγt/RORα (n=4) and Id2iΔRORγt/Tbet (n=2) mice (Data are representative of 3 independent experiments). f Mapping of the reads detected by scRNA-seq along the Rorc locus in tdRFP+ ILCs from SILP of Id2creERT2, Id2iΔRORγt, Id2iΔRORγt/RORα and Id2iΔRORγt/Tbet mice. The localisation of LoxP-flanked exons is depicted by a grey box. Each data point on graphs is a mouse, horizontal bars in ‘b’ denote the median, bars in ‘d’ and ‘e’ denote the mean, with error bars denoting the SEM. Statistical significance in ‘b’ was tested using an ordinary one-way ANOVA with Dunnett’s test for multiple comparisons. ****P ≤ 0.0001.
