Extended Data Fig. 5: Related to Fig. 4.
Extended Data Figure 5a, comparison of motif position weight matrices as defined from de novo motif discovery of pre-positioned and GCB de novo sites. Motif divergence was computed using the R package motifDiverge. Significant divergence indicated by *. b, results of de novo motif discovery as reported by HOMER for OCT2 ChIP-seq and OCAB ChIP-seq in GCBs. c, barplot showing the fraction of sites with OCAB recruitment in mouse GCBs and comparing sites with OCT2 direct binding in murine splenic B cells and sites in which OCT2 is absent. ChIP-seq data for OCT2 and OCAB were retrieved from GSE142040. P-value by Fisher’s exact test. d, boxplots of log2 fold changes in mouse GCB (n = 10) vs NB (n = 4) chromatin accessibility, mouse GCB (n = 2) vs NB (n = 2) histone H3K27ac, and mouse GCB (n = 2) vs NB (n = 2) histone H3K4me1 and comparing OCT2 direct sites in splenic B cells that recruit OCAB in GCBs (OCT2-OCAB sites) with all other accessible sites. P-values by Welch t-test. Box plots show center line as median, box limits as upper and lower quartiles, whiskers as minimum and maximum values within 1.5 x inter-quartile range of 1st and 3rd quartile, and notches as approximate 95% CI of the median.
