Extended Data Fig. 6: Related to Fig. 4.

Extended Data Figure 6a, illustration of the strategy for OCAB depletion (OCABi) in GCB-derived cells. Viable, GFP+ OCI-Ly7 and SUDHL4 cells expressing dCas9-KRAB were isolated by FACS 72 hours after transduction with sgRNA and GFP. b, chromatin accessibility PCA of B cells and cell lines using DAEs across B cell phenotypes. c, gene expression PCA of B cells and cell lines using differentially expressed genes across B cell phenotypes (FDR < 0.001). d, plot showing the fraction of GFP+ viable OCI-Ly7 or SUDHL4 (e) cells after transduction with control sgRNAs (n = 6) or sgRNAs targeting OCAB (OCABi, n = 15). Shaded area shows 95% CI of the mean. f, bar plot showing OCAB transcript abundance (TPM)⁵¹ in OCABi (n = 6 replicate experiments) vs control (n = 6) OCI-Ly7 cells, and OCABi (n = 2) vs control (n = 2) SUDHL4 cells. P-values are calculated by Welch t-test and error bars show standard error of the mean. g, OCAB expression relative to HPRT in control (n = 3) and OCABi (n = 3) OCI-Ly7 cells by qPCR. P-values are calculated by Welch t-test and error bars show standard error of the mean. h, unsupervised hierarchical clustering dendrogram based on Pearson correlation distance of OCABi and control OCI-Ly7 and SUDHL4 chromatin accessibility and gene expression (i). j, scatter plot showing chromatin accessibility of OCABi and control OCI-Ly7 or SUDHL4 (k) cells projected onto PCs from primary B cell chromatin accessibility PCA (Fig. 4I). l, scatter plot showing OCI-Ly7 OCABi and control or SUDHL4 OCABi and control (m) gene expression projected onto PCs from primary B cells (Fig. 4K). n, illustration showing trajectories in B-cell development during the humoral immune response. Relative position of cell types and length of arcs is defined from PCA of mean accessibility signal for each cell type.