Extended Data Fig. 7: Related to Fig. 5. | Nature Immunology

Extended Data Fig. 7: Related to Fig. 5.

From: OCT2 pre-positioning facilitates cell fate transition and chromatin architecture changes in humoral immunity

Extended Data Fig. 7

Extended Data Figure 7a, experimental design for forced expression of Pou2af1, the gene encoding OCAB in mouse. Splenic B cells from n = 5 mice were stimulated with LPS ex vivo and transduced with a GFP vector containing Pou2af1 (mOCAB) or GFP only (control). b, expression of Pou2af1 in mOCAB (n = 5) and control(n = 5) B cells. Expression levels were measured by qPCR using cDNA generated from total RNA of GFP+ viable cells and normalized to that of Hprt1, calculated by the delta-delta Ct method (2−ΔΔCtand represented as a fold change over the mean expression in control cells. The expression of each gene was measured in triplicate across 5 replicates. P-values are calculated by Welch’s t test and error bars show standard error of the mean.

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