Extended Data Fig. 8: Thymic CD4+ CD8+ compartments are not restored in RORγt/T-bet double-deficient mice. | Nature Immunology

Extended Data Fig. 8: Thymic CD4+ CD8+ compartments are not restored in RORγt/T-bet double-deficient mice.

From: T-bet and RORα control lymph node formation by regulating embryonic innate lymphoid cell differentiation

Extended Data Fig. 8

a, Mapping of reads detected by bulk RNA-seq to the mm10 mouse Rorc locus in indicated mouse strains. Red box designates localization of transcriptional start site (TSS) ATG of exon 1γt. (b) Expression of Rorc exon1-3 and Rorc exon5-6 junctions in small intestinal LinLD−CD45+ CD3− GFP+ ILCs of 4-week-old mice determined by quantitative PCR. Values are normalized to housekeeping gene Gapdh. Each symbol represents an individual mouse. Data show mean ± SEM, reporter and DKO n = 6, RKO n = 2 examined over 2 independent experiments. (c,d,e) Representative flow cytometry plots of indicated populations and compartments isolated from 4-week-old mice. (f) Violin plot of Rora expression within ILCP of designated mouse strains from E18.5 scRNA-seq dataset. Detailed statistics are available in source data.

Source data

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