Extended Data Fig. 7: Lineage-specific BMAL1-deficiency migration analysis.
From: Circadian clocks guide dendritic cells into skin lymphatics
a, b, Representative whole mount staining for PROX-1 and VE-CADHERIN in mouse EAR skin. Dotted lines represent a LYVE-1+ capillary. Scale bars, 50 µm and 10 µm for higher magnification. c, d, 6 h endogenous crawl-in assays in EC-specific (Bmal1ΔEC; c) and LEC-specific (Bmal1ΔLEC; d) Bmal1-/- animals and controls. Values presented as intravascular versus extravascular ratio of CD11c+ cells. Representative images show a 24 h endogenous crawl-in assay, stained for CD11c and LYVE-1, scale bar, 50 µm, two-way ANOVA with Šidák post test, n = (c, WT) 5,4,3,5; (c, KO) 4,3,4,4); (d, WT) 4,4,5,4; (d, KO) 7,7,5,5) mice from 2 independent experiments. All data are represented as mean ± s.e.m.
