Extended Data Fig. 3: LEC protein analysis.
From: Circadian clocks guide dendritic cells into skin lymphatics
a, b, LYVE-1+ staining overviews of skin, small intestine (SI), inguinal lymph node (iLN) and lung tissues used for quantitative analysis. Scale bar, 150 µm, 100 µm for skin, representative images of 2 independent experiments, a. b, Respective sub-structures and higher magnifications for analysis. Schematic view of the iLN marks lymphatic sinusoids. CS = cortical sinus, MS = medullary sinus, SCS = subcortical sinus, SMLV = sub-mucosal LV, representative images of 2 independent experiments. Scale bar, 50 µm. c–g, Quantitative immuno-fluorescence microscopy screen of proteins on LVs in mouse lung, c, iLN, d, e, SI, f, and skin, g, sections as well as sections from human skin biopsies, h. * = one-way ANOVA, # = cosinor analysis, n = 5 for murine (data are representative of 2 independent experiments) and 7,12,7,5 (CD99) and 5,8,4,7 (JAM-A) for human samples. Dotted line represents fit curves. All data are represented as mean ± s.e.m.
