Extended Data Fig. 4: LEC mRNA and DC protein analysis.
From: Circadian clocks guide dendritic cells into skin lymphatics
a, Gating strategy of sorted dermal CD31+ PODOPLANIN+ lymphatic endothelial cells (LECs) for qPCR and RNA-sequencing analyses. b, Relative mRNA expression of Cd99 in sorted dermal LECs after qPCR analysis, one-way ANOVA with Tukey’s post-test, n = 3 mice, data are representative of 2 independent experiments. c, Expression of LEC, blood endothelial cell (BEC), leukocyte (leu) and stromal cell (SRC)-specific genes in sorted dermal LECs presented as normalized counts and analysed by RNA sequencing, one-way ANOVA, n = 20. d, Clock gene expression of sorted dermal LECs across 4 time points measured and depicted as normalized counts. * = one-way ANOVA, # = cosinor analysis, n = 5 mice. e, f, Flow-cytometric analysis of CCR7 on emigrated DCs after a 24 h crawl-out assay. e, MFI of CCR7 on Langerhans cells (LCs), n = 9 mice from 2 independent experiments. f, Histograms of CCR7 MFI on emigrated cDCs (EPCAM-) and LCs (EPCAM+) in comparison to an isotype control, unpaired student’s t-test. All data are represented as mean ± s.e.m.
