Extended Data Fig. 4: STAT3 is required for CM-induced protumorigenic polarization.
a, Immunoblots of indicated proteins in BMDMs expressing scramble or STAT3-targeting gRNAs treated with 10 ng/ml IL-6 for 6 h. Data are representative results of two independent experiments. b, c, Immunoblots of indicated proteins (b) and qPCR analysis of mRNA expression of indicated genes (c) in BMDMs treated with CM in the presence of vehicle (CM) or 10μM Stattic (CM + Stattic) (n = 6 for Ctrl and n = 5 for CM and CM + Stattic). Data are representative results of three independent experiments. d, Multiplex cytokine array was used to determine the concentration of IL-10 (left) and IL-6 (right) in CM from YUMM1.7 and MEF cells. Stand0 to Stand7 show the increased concentration detected by the standard provided by the kit (n = 4). Data are representative results of two independent experiments. e, Immunoblot of indicated proteins in BMDMs treated with control media (Ctrl), cancer cell conditioned media (CM) or CM plus 50μM STF081030 for 18 h. Data are representative of two independent experiments. f, Immunoblot of BMDMs treated with control vehicle (Ctrl), cancer cell conditioned media (CM) or tunicamycin (1μg/ml; Tuni.) for the indicated time points. Data are representative of two independent experiments. All data are mean ± s.e.m and were analyzed by two-tailed, unpaired Student’s t-test.
