Extended Data Fig. 1: TAMs display high lipid content and ER stress responses in the inducible Braf/Pten melanoma model.
a, Representative histogram (left) and quantitative plot (right) of BODIPY C12 staining in splenic macrophages (n = 10) or tumor-associated macrophages (TAMs) (n = 11) from YUMM1.7 melanoma bearing mice. Data are pooled from two independent experiment. b–h, Analysis of Braf/Pten tumor-bearing mice seven weeks after tamoxifen administration. b, c, Representative histogram (left) and quantitative plot (right) of BODIPY staining (b) and BODIPY C12 staining (c) in splenic macrophages or TAMs isolated from Braf/Pten tumor-bearing mice (n = 5 per group). d, Representative histogram (left) and quantitative plots (right) of BODIPY staining in TAMs gated based on ARG1 expression (n = 10 per group). e, qPCR analysis of mRNA expression of the indicated genes in splenic macrophages and TAMs isolated from Braf/Pten tumor-bearing mice (n = 4 per group). f, Representative histogram (left) and quantitative plot of the abundance (right) of sXBP1+ subset among splenic macrophages and TAMs in Braf/Pten tumor-bearing mice (n = 10 per group). g, Representative histogram (left) and quantitative plot (right) of BODIPY staining in TAMs gated based on sXBP1 expression (n = 10 per group). h, Representative histogram (left) and quantitative plot of the abundance (right) of sXBP1+ cells among ARG1+ and ARG1− TAMs (n = 10 per group). i, Gating strategy applied to define ARG1 + macrophages from spleen and tumor of YUMM1.7 tumor-bearing mice. j–l, Representative histogram (j) and quantitative plot of BODIPY staining (k) or sXBP1 (l) in skin-resident macrophages or TAMs from Braf/Pten tumor-bearing mice (n = 10 per group). m, Representative histogram (left) and quantitative plot (right) of pPERK staining in splenic macrophages or tumor-associated macrophages (TAMs) (n = 9 per group) of YUMM1.7 melanoma bearing mice. Data are pooled from two independent experiments. n, qRT-PCR of the indicated genes from sorted splenic macrophages and TAMs (n = 8 per group) isolated from YUMM1.7 tumor-bearing mice. Data are representative of two independent experiments (b, c, e). Data are pooled from two independent experiment (a, d, f–h, j–n). Each symbol represents one individual. All data are mean ± s.e.m and were analyzed by two-tailed, unpaired Student’s t-test or paired t-test (d, g, h).
