Extended Data Fig. 2: Specific reduction of SDHA activity in colon and ileum post allo-HCT.
From: Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology
C57BL/6 animals received 10 Gy total body irradiation and received 3×106 (BALB/c → C57BL/6) or 1 ×106 (C3H.SW → C57BL/6) T cells along with 5×106 TCD-BM cells from either syngeneic or allogeneic donors. (a) Representative images of SDH enzyme activity staining in GVHD target tissues (colon, ileum, liver and skin) and non-target tissues (heart, pancreas and kidney) from naive animals or recipients 21days post HCT (BALB/c → C57BL/6,). (b) Representative images of SDH enzyme activity staining in GVHD target tissues (colon, ileum) from recipients 21days post HCT (C3H.SW → C57BL/6). (c) Integrated intensity of SDH enzyme activity staining from colon and ileum 7days post HCT (BALB/c → C57BL/6, n = 5 biologically independent animals). Representative plots and a graph summarizing the results of at least two independent experiments are shown. Scale bar (a,b): yellow=500 µm, black=200 µm. All statistical analysis by two-tailed Mann-Whitney test (c) (mean ± s.e.m.).
