Extended Data Fig. 1: Tetramer-positive CD8+ T cells from a mixture of pMHC tetramer-sorted polyclonal T cells cultured in vitro and quality check of gene expression.
From: High-throughput and high-dimensional single-cell analysis of antigen-specific CD8+ T cells
a, Gating strategy for sorting tetramer-positive CD8+ T cells from a mixture of pMHC tetramer sorted polyclonal T cells cultured in vitro. b,c, Distribution of mRNA counts (log10) (b) and number of detected genes per cell (c) among different antigen-specific T cell populations. Horizontal lines represent 25th percentile, median and 75th percentile values, with whiskers extending to the farthest data point within a maximum of 1.5 × interquartile range.
