Extended Data Fig. 3: TetTCR-SeqHD enables combined gene expression, phenotype and TCR clonality comparison among antigen-specific CD8+ T cells.
From: High-throughput and high-dimensional single-cell analysis of antigen-specific CD8+ T cells
a, UMAP of single cells among different donors. Grey dots represent all cells and colored dots are cells from different chips. b, Comparison of the distribution of phenotypes between tetramer-positive and tetramer-negative CD8+ T cells. c, Percentage of naive population in each antigen-specific CD8+ T cell group. d, Percentage breakdown of naive CD8+ T cells among all major antigen specificities. Only antigen specificities with a percentage greater than 1% within the naive population are shown. ‘NEG’ cells are tetramer-negative CD8+ T cells identified by tetramer MID counts. Cells were classified into filter category based on the following criteria: (1) more than one antigen binds to a single cell, and these antigens are a distance of more than 3 amino acids away from each other; (2) correlation of tetramer MID between single cells and the median of all cells with same TCR sequence is below 0.9, identified as described in Methods.
