Extended Data Fig. 4: Distribution of representative tetramer MID counts.
From: High-throughput and high-dimensional single-cell analysis of antigen-specific CD8+ T cells
a, Distribution of tetramer MID counts for eight antigen specificities, including EBV antigens (EBV-BLMF1, BZLF1-190-197, EBV-BRLF1 and EBV-LMP2A), influenza viral antigens (M1 and NP44-52), T1D-associated antigens (PTPRN-FGD-9 and ZNT8-115-123) or cross-reactive antigens (HCV, Mart1 and DUF5119-124-133/INSDRIP-1-9/PTPRN-797-805). For each cell in the group, the MID counts for each of the 280 antigens used in the experiment were tallied and then overlaid in the same order of the 280 antigens. Only the antigens that emerge after the filter are labeled on the x axis, and their position in the 280 antigen list is indicated by a tick on the x axis. Each panel with a sharp single peak indicated single antigen specificity, while panels with multiple sharp peaks indicated cross-reactive antigens. b, Comparison of tetramer MID counts among DUF5119-124-133/INSDRIP-1-9/PTPRN-797-805 cross-reactive and single antigen-specific cells.
