Extended Data Fig. 8: Klf4 is controlled by Bmal1 and drives circadian rhythmicity of phagocytosis.
From: Aging disrupts circadian gene regulation and function in macrophages
a-d. Circadian RT-PCR expression patterns of Klf4 (a-b), and the phagocytosis-related genes Gba (c) and Rab3d (d) reveal loss of rhythmicity of phagocytosis genes in Klf4-shRNA lentiviral injected young mice as compared to scrambled vector controls. Data are mean ± s.e.m, n = 18 mice in each treatment group and n = 3 in each time group. Indicated p-values were calculated by JTK_CYCLE. e-f. RT-PCR expression of Bmal1 (e) and Circadian RT-PCR expression patterns of Klf4 in Bmal1-shRNA lentiviral injected young mice as compared to scrambled vector controls. Data are mean ± s.e.m, n = 18 mice in each treatment group and n = 3 in each time group. Indicated p-values were calculated by JTK_CYCLE. g. Phagocytosis of fluorescent E. coli particles by Bmal1-shRNA lentiviral and scrambled vector injected young mice. Data are mean ± s.e.m, n = 18 mice in each treatment group and n = 3 in each time group. Indicated p-values were calculated by JTK_CYCLE. h. Crystal structure of the zinc-finger domain of KLF4 in complex with DNA and rs2236599 synonymous mutation site predicted by SWISS-MODEL57.
