Extended Data Fig. 7: Somatic hypermutation frequencies of SARS-CoV-2 S-specific B cells.
From: BNT162b2 vaccine induces divergent B cell responses to SARS-CoV-2 S1 and S2
a-e, Single-cell BCR repertoire sequencing data showing SHM frequencies for IGHV (left) and IGLV (right) of B cells specific for a, S2 (n = 210 cells), b, RBD (n = 124 cells) and c, S1n (n = 21 cells), as determined by barcoded S-antigen-tetramers at three timepoints post vaccination. d-e, SHM frequencies of B cells that were recombinantly expressed as monoclonal antibodies specific for d, S1 (high, n = 17 cells; low, n = 18 cells) and e, S2 (high, n = 8 cells; low, n = 6 cells), corresponding to (Fig. 4a). High binding monoclonal antibodies are defined by the data shown in (Fig. 4a) as positive for both PE and APC barcode tetramer binding and ELISA AUC > 3. Individual values, means and standard deviations are shown. Exact p-values according to two-tailed one-way Kruskal Wallis test followed by Dunnett’s multiple comparison test (a-c), and unpaired two-tailed Student’s t-test (d, e). Immunoglobulin heavy-V gene (IGHV), immunoglobulin light-V gene (IGLV), somatic hypermutation (SHM), monoclonal antibodies (mAbs).
