Extended Data Fig. 2: Clonal and transcriptomic B cell responses to mRNA vaccination.
From: BNT162b2 vaccine induces divergent B cell responses to SARS-CoV-2 S1 and S2
a-k, Single-cell transcriptome and BCR repertoire sequencing data showing a, cells corresponding to clonal families shown in (Fig. 2a). Number of cells in each B cell cluster for each clonal family (n = 106 clonal families) at all four timepoints. Statistics are not calculated for day 0 because clonal families are selected to contain at least one clone at day 0 in the U-Bmem cell cluster. b, Proportions of cells (n = 476 cells) in each cluster from clonal families containing U-Bmem cells at day 0 for all four timepoints. c, Average SHM frequency of clonal U-Bmem cell families at day 0 and clonal SR-Bmem cell families at days 7-28. Each dot represents the average of each mutation frequency for one clonal family. d, Cells corresponding to the clonal families shown in (Fig. 2d). Number of cells in each clonal family (n = 52 clonal families) in each B cell cluster at all four timepoints. Statistics are not calculated for D28 because clonal families are selected to contain at least one clone at D28 in the plasmablast cluster. e, Proportions of cells (n = 274 cells) in each cluster from clonal families with a plasmablasts at D28 for all four timepoints. f-h, Gene enrichment signatures of differentially expressed genes via Metascape in f, U-Bmem cells, g, SR-Bmem cells and h, plasmablasts for day 7, day 21 and day 28 versus day 0. Dashed line represents q < 0.01. j,k, Volcano plots of differentially expressed genes in j, U-Bmem cells and k, SR-Bmem cells at timepoints day 7 (left), day 21 (center), and day 28 (right) relative to day 0. a, d, Individual values with mean and standard errors of the mean are shown. c, Individual values with mean and standard deviations are shown. Exact p-values according to a,d, two-tailed Kruskal-Wallis test, followed by Dunnett’s multiple comparison test and c, paired two-tailed t test. Memory B cells (Bmem cells), resting switched memory B cells (SR-Bmem cells), activated switched memory B cells (SA-Bmem cells), double-negative B cells (Bdn cells), naive B cells (Bnaive cells), immunoglobulin heavy-V gene (IGHV), immunoglobulin light-V gene (IGLV), somatic hypermutation (SHM).
